Garrick RC, Meadows CA, Nicolas AN, Nason JD, Dyer RJ. 2008. A set of polymorphic nuclear intron markers for conservation genetics and phylogeography of Euphorbia species (Pedilanthus clade). Conservation Genetics, 9 1673-1676.

We developed seven nuclear intron markers for Euphorbia lomelii. New exon-primed intron-crossing (EPIC) oligonucleotides were used for initial amplification and sequencing, then locus-specific primers and restriction fragment length polymorphism genotyping assays were designed. Loci showed no significant deviation from Hardy–Weinberg and linkage equilibrium, and they cross-amplify in at least three congeneric species.

DOI: 10.1007/s10592-008-9521-5

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